原创力文档the effects of hiv on the sensitivity of a whole blood ifn-γ release assay in zambian adults with active tuberculosis艾滋病毒的敏感性的影响全血ifn-γ释放试验在赞比亚患有活动性结核病.pdfVIP
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the effects of hiv on the sensitivity of a whole blood ifn-γ release assay in zambian adults with active tuberculosis艾滋病毒的敏感性的影响全血ifn-γ释放试验在赞比亚患有活动性结核病
The Effects of HIV on the Sensitivity of a Whole Blood
IFN-c Release Assay in Zambian Adults with Active
Tuberculosis
1 2 1 2 1,2 1,2
Edward Raby *, Maureen Moyo , Akash Devendra , Joseph Banda , Petra De Haas , Helen Ayles ,
Peter Godfrey-Faussett1
1 Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom, 2 The Zambia AIDS Related TB (ZAMBART)
Project, Department of Microbiology, University Teaching Hospital, Lusaka, Zambia, 3 The Zambia AIDS Related TB (ZAMBART) Project, Department of Pathology,
University Teaching Hospital, Lusaka, Zambia, 4 The Zambia AIDS Related TB (ZAMBART) Project, Department of Medicine, University Teaching Hospital, Lusaka, Zambia
Abstract
Background: Interferon gamma release assays (IGRA) are replacing the tuberculin skin test (TST) as a diagnostic tool for
Mycobacterium tuberculosis infection. However research into the test’s performance in the high HIV-TB burden setting is
scarce. This study aimed to define the sensitivity of an IGRA, QuantiFERON-TBH Gold In-Tube (QGIT), in adult Zambian
patients with active smear-positive tuberculosis. Secondary outcomes focussed on the effect of HIV on the test’s
performance.
Principal Findings: Patients attending government health clinics were recruited within 1 month of starting treatment for TB.
Subjects were tested with QGIT and TST. T lymphocyte counts were estimated (CD3+ + +
, CD4 , CD8 ). QGIT was performed for
112 subjects. 83/112 were QGIT p
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