the human nucleolar protein ftsj3 associates with nip7 and functions in pre-rrna processing人类的核仁的蛋白质ftsj3 associates在pre-rrna nip7和功能处理.pdfVIP

The Human Nucleolar Protein FTSJ3 Associates with NIP7 and Functions in Pre-rRNA Processing 1,4 2 3 1 Luis G. Morello , Patricia P. Coltri , Alexandre J. C. Quaresma , Fernando M. Simabuco , Tereza C. L. 1 4 3 2 4 5 Silva , Guramrit Singh , Jeffrey A. Nickerson , Carla C. Oliveira , Melissa J. Moore , Nilson I. T. Zanchin * ´ ˆ ˜ ˜ 1 Laboratorio Nacional de Biociencias, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, Sao Paulo, Brazil, 2 Department of Biochemistry, University of Sao ˜ Paulo, Sao Paulo, Brazil, 3 Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America, 4 Department of Biochemistry and Molecular Pharmacology and Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, Massachusetts, ˜ ´ United States of America, 5 Instituto Carlos Chagas, Fundac¸ao Instituto Oswaldo Cruz, Curitiba, Parana, Brazil Abstract NIP7 is one of the many trans-acting factors required for eukaryotic ribosome biogenesis, which interacts with nascent pre- ribosomal particles and dissociates as they complete maturation and are exported to the cytoplasm. By using conditional knockdown, we have shown previously that yeast Nip7p is required primarily for 60S subunit synthesis while human NIP7 is involved in the biogenesis of 40