c-terminal extension of the yeast mitochondrial dna polymerase determines the balance between synthesis and degradation酵母线粒体dna聚合酶c端扩展的决定之间的平衡合成和降解.pdfVIP

C-Terminal Extension of the Yeast Mitochondrial DNA Polymerase Determines the Balance between Synthesis and Degradation Katrin Viikov, Olga Jasnovidova, Tiina Tamm, Juhan Sedman* Department of Biochemistry, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia Abstract Saccharomyces cerevisiae mitochondrial DNA polymerase (Mip1) contains a C-terminal extension (CTE) of 279 amino acid residues. The CTE is required for mitochondrial DNA maintenance in yeast but is absent in higher eukaryotes. Here we use recombinant Mip1 C-terminal deletion