design and characterization of auxotrophy-based amino acid biosensorsauxotrophy-based氨基酸生物传感器的设计和描述.pdfVIP

Design and Characterization of Auxotrophy-Based Amino Acid Biosensors Felix Bertels, Holger Merker, Christian Kost* Research Group Experimental Ecology and Evolution, Max Planck Institute for Chemical Ecology, Jena, Germany Abstract Efficient and inexpensive methods are required for the high-throughput quantification of amino acids in physiological fluids or microbial cell cultures. Here we develop an array of Escherichia coli biosensors to sensitively quantify eleven different amino acids. By using online databases, genes involved in amino acid biosynthesis were i