detailed enzyme kinetics in terms of biochemical species study of citrate synthase详细的酶动力学的生化物种柠檬酸合成酶的研究.pdfVIP

Detailed Enzyme Kinetics in Terms of Biochemical Species: Study of Citrate Synthase Daniel A. Beard*, Kalyan C. Vinnakota, Fan Wu Biotechnology and Bioengineering Center and Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America Abstract The compulsory-ordered ternary catalytic mechanism for two-substrate two-product enzymes is analyzed to account for binding of inhibitors to each of the four enzyme states and to maintain the relationship between the kinetic constants and the reaction equilibrium constant. The develo