detection and removal of biases in the analysis of next-generation sequencing reads分析检测和消除偏见的下一代测序读.pdfVIP

Detection and Removal of Biases in the Analysis of Next- Generation Sequencing Reads Schraga Schwartz.¤a*, Ram Oren.¤b¤c¤d, Gil Ast Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel-Aviv University, Ramat Aviv, Israel Abstract Since the emergence of next-generation sequencing (NGS) technologies, great effort has been put into the development of tools for analysis of the short reads. In parallel, knowledge is increasing regarding biases inherent in these technologies. Here we discuss four different biases we encountered while