dissection of splicing regulation at an endogenous locus by zinc-finger nuclease-mediated gene editing解剖剪接调控的内在轨迹由锌指nuclease-mediated基因编辑.pdfVIP

Dissection of Splicing Regulation at an Endogenous Locus by Zinc-Finger Nuclease-Mediated Gene Editing Sandra Cristea, Philip D. Gregory, Fyodor D. Urnov, Gregory J. Cost* Sangamo BioSciences, Richmond, California, United States of America Abstract Sequences governing RNA splicing are difficult to study in situ due to the great difficulty of traditional targeted mutagenesis. Zinc-finger nuclease (ZFN) technology allows for the rapid and efficient introduction of site-specific mutations into mammalian chromosomes. Using a ZFN pair