聚合酶链反应快速检出耐甲氧西林凝固酶阴性葡萄球菌的研究(Rapid detection of methicillin resistant coagulase negative staphylococci by polymerase chain reaction).docVIP
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聚合酶链反应快速检出耐甲氧西林凝固酶阴性葡萄球菌的研究(Rapid detection of methicillin resistant coagulase negative staphylococci by polymerase chain reaction)
聚合酶链反应快速检出耐甲氧西林凝固酶阴性葡萄球菌的研究(Rapid detection of methicillin resistant coagulase negative staphylococci by polymerase chain reaction)
Rapid detection of methicillin resistant coagulase negative staphylococci by polymerase chain reaction
2009-10-17 Mo LAN, Wang Qinan
[Abstract] objective to rapidly detect methicillin resistant coagulase negative staphylococci. By using the method of polymerase chain reaction (PCR) detection of methicillin resistant coagulase negative staphylococcus, Staphylococcus from establishing a rapid DNA extraction method, the crude extract used as template, the detection of methicillin-resistant Staphylococcus aureus encoding penicillin binding protein of mecA gene, amplified 533bp size. Results the drug sensitive method identified 21 strains of methicillin resistant coagulase negative staphylococci, 100% mecA gene positive, 23 strains of methicillin sensitive coagulase negative staphylococci, and 9 mecA fragments were amplified by PCR. R-32P blot was used as a probe, and the results of Southern hybridization were identical with that of PCR. It was proved that the 533bp fragment was a specific fragment of mecA gene of methicillin resistant coagulase negative staphylococci. Conclusion PCR method is rapid and sensitive, and it can detect critical (MIC=0.5 ~ 2mg/L) methicillin resistant coagulase negative staphylococci strains.
[Keywords] coagulase negative staphylococci polymerase chain reaction gene, mecA
Rapid detection of methicillin-resistant coagulase-negative staphylococci by using the polymerase chain reaction Mo Lan, Wang Qinan. Department of Infectious Diseases, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016
[Abstract] Objective In this study, a simple and reliable method using PCR was devised to identify methicillin-resistant CNS (coagulase-negative staphylococci, CNS Methods A rapid cell). Lysis procedure was established for the release of DNA from staphylococci. Using the crude extract of the strain as a temp
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