烘烤过程中烤烟细胞壁生理变化研究-中国烟草科学.pdf

中国烟草科学 2010 ，31 （3 ）：73-77 73 烘烤过程中烤烟细胞壁生理变化研究 1 1 1 2 1 1 1* 武圣江 ，宋朝鹏 ，许自成 ，王松峰 ，李富强 ，程传策 ，宫长荣 （1.河南农业大学烟草学院，郑州 450002 ；2. 农业部烟草类作物质量控制重点实验室，中国农业科学院烟草研究所， 青岛 266101 ） 摘 要：采用电热式温湿自控密集烤烟箱对烤烟上部烟叶在烘烤中细胞壁水解酶和主要组分随温湿度的变化进行了研究。 结果表明：果胶甲酯酶(PME)、多聚半乳糖醛酸酶(PG)和纤维素酶在38 ～48 ℃酶活性较高。相关性分析表明：PME 与纤维 素酶呈极显著正相关。烘烤中可溶性果胶含量不断增加，原果胶、总果胶和纤维素含量不断降低，其中果胶(总果胶、原果 胶及可溶性果胶)与PG 的关系比果胶与PME 的关系更密切。 关键词：密集烘烤；光滑烟；细胞壁；酶；组分 中图分类号：S572.01;TS441 文章编号：1007-5119（2010 ）03-0073-05 DOI: 10.3969/j.issn.1007-5119.2010.03.017 Cell Wall Physiological Changes of Tobacco Leaves during Curing Process 1 1 1 2 1 1 WU Shengjiang , SONG Zhaopeng , XU Zicheng , WANG Songfeng , LI Fuqiang , CHENG Chuance , 1* GONG Changrong (1.College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China; 2.Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute of CAAS, Qingdao 266101, China) Abstract: The change pattern of major components and hydrolase in the cell wall of upper tobacco leaves with temperature and humidity during the bulk flue-curing process was studied by using the electric-heated flue-curing barn designed by Henan Agricultural University. The results showed that pectin methylesterase (PME), polygalacturonase (PG) and cellulase were highly active at 38 to 48 oC. Correlation between PME and cellulase was highly positive significant. During curing, leaf soluble pectin content increased continu