APC基因异常甲基化在上皮性卵巢癌中检测及临床意义.docVIP

APC基因异常甲基化在上皮性卵巢癌中检测及临床意义 　 作者：孙健 卢健 邹冬芳 吴国平 【摘要】 目的 观察结肠腺瘤性息肉病 (APC) 基因异常甲基化在上皮性卵巢癌中的作用及临床意义。方法 59例上皮性卵巢癌组织原发灶，32例相应的盆、腹腔转移灶，12例癌旁卵巢组织及30例正常卵巢组织，采用甲基化特异性聚合酶链式反应(MSP)法检测APC基因启动子区甲基化状态。结果 上皮性卵巢癌组织原发灶及相应的盆、腹腔转移灶中存在APC基因启动子区异常甲基化，发生率分别为32.2%、40.6%，显著高于正常卵巢组织(Plt;0.01)。12例癌旁卵巢组织中1例检测到APC基因启动子区甲基化，30例正常卵巢组织未检测到。APC基因启动子区甲基化的发生率在临床Ⅰ、Ⅱ期低于Ⅲ、Ⅳ期(Plt;0.05)，在高分化癌中低于低分化癌(Plt;0.05)。结论 APC基因启动子区异常甲基化与上皮性卵巢癌发生密切相关，并可能与上皮性卵巢癌临床分期及分化程度有关。 【关键词】 卵巢癌； APC基因； DNA甲基化 【Abstract】 Objective To observe the role of promoter hypermethylation of adenomatous polyposis coli (APC) gene in the course of epithelial ovarian carcinoma. Methods Hypermethylation of APC promoter was detected by methylation specific PCP (MSP) in 59 epithelial ovarian cancer primary tissues, 32 metastatic tissues of pelvic and abdomen cavity, 12 adjacent noncancerous ovarian tissues and 30 normal ovarian tissues. Results Hypermethylation of APC promoter was detected in ovarian cancer tissues and metastatic sites,the frequency being 32.2% and 40.6% respectively, which was significantly higher than that in normal ovarian tissues(Plt;0.01).Hypermethylation of APC promoter was detected in 1 out of 12 adjacent noncancerous ovarian tissues,and no hypermethylation was detected in normal ovarian tissues.The frequency of hypermethylation of APC promoter was significantly lower in cancers of stage I and Ⅱ than that in stage Ⅲ and IV (Plt;0.05),and so was that in well differentiated cancers than that in poorly differentiated ones. Conclusion Promoter hypermethylation of APC plays an important role in the course of epithelial ovarian carcinoma, and it is related to clinical stage and histopathological grade in epithelial ovarian carcinoma. 【Key words】 Ovarian carcinoma；APC gene；DNA hypermethylation 结肠腺瘤性息肉病 (adenomatous polyposis coli，APC) 基因是一种抑癌基因，该基因启动子区CpG岛甲基化可使APC蛋白缺失。近来发现在结直肠癌、乳腺癌、肺癌、子宫内膜癌、食管癌、膀胱癌、胃癌、T淋巴细胞瘤等肿瘤中均有APC基因超甲基化［1］，然而对上皮性卵巢癌中APC基因甲基化相关的系统分析还少见报道。我们以上皮性卵巢癌为对象，用甲基化特异性聚合酶链反应(methylationspecific PCR，MSP)法对APC基因甲基化进行