不同糖浓度对体外培养小鼠成纤维细胞增殖、分化影响.docVIP

不同糖浓度对体外培养小鼠成纤维细胞增殖、分化影响 　作者：李盛，石汉平，孙冠青，孟锐 【摘要】 　　 【关键词】 创伤愈合 成纤维细胞 血糖 应激反应 　　Abstract： Objective To find the relationship between glucose concentration and the proliferation of fibroblast.Methods The proliferation of mouse fibroblast was tested in different concentration of glucose at 12h,24h,48h with CCK kit.The amount of the outcell type I collagen fibers was tested by the mouse type I collagen Elisa kit,and the expression of αsmooth muscle actin (αSMA) tested by immunochemistry.Results We found that under the medium of low concentration,the proliferation was much more than that in the high concentration groups after 12h,24h and 48h(Plt;0.01),namely the concentration was negatively correlated with the proliferation.We tested the outcell type I collagen fibers using the mouse type I collagen Elisa kit,but there was no difference between each group(Pgt;0.05).About the expression of mouse αSMA,positive correlation was found between the glucose concentration and the expression of the mouse αSMA.Conclusion At the original augmentation of the blood glucose,the proliferation of fibroblast is not different.But when it gets over a level,the proliferation will be depressed,which can lead to the delay of the healing of raw surface；the concentration of glucose has nothing to do with the outcell type I collagen fibers；the concentration of glucose is a factor which enhances the variation from fibroblast to myofibroblast,and the concentration of glucose is positively correlated with the tendency of the variation. 　　Key words：wound healing;fibroblast;blood glucose;stress reaction 　　在外科危重患者中常常遇到高血糖，其中主要是存在许多糖尿病患者，糖尿病损伤修复时，损伤组织的高糖环境对成纤维细胞的生长有着很大影响，使得创面愈合这一过程变得更为复杂。血糖持续升高可使机体产生一系列的不良反应，增加机体高渗性昏迷、糖尿病酮症酸中毒发生的可能性， 严重危及生命。高血糖的发生提示机体处于代谢紊乱状态， 其持续存在更增加了感染的风险。因此如何应对创伤伴随高血糖并发症的出现，一直是创伤外科亟待解决的问题。 　　创伤愈合是一个复杂而有序的生物学过程，涉及炎症反应、细胞增殖、迁移，创面重塑等多个序贯又相互交叉的病理生理过程。研究已发现成纤维细胞（fibroblast）在此修复过程中起着十分重要的作用。长期以来它也一直被视为是肉芽组织收缩的能量供应者，对伤口有效而迅速的关闭起着重要的作用。肌成纤维细胞被认为是由创口周围的皮肤、皮下组织中的成纤维细胞在各种刺激因子的作用下