吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤保护作用.docVIP

吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤保护作用 　 作者：孟素峰，李有清，王启明 【摘要】 目的 观察吡咯烷二硫氨基甲酸酯(PDTC)对体外循环(extracorporeal circulation,ECC)中犬心肌线粒体损伤的保护作用。方法 12只犬随机分为对照组(C组,n=6)和PDTC组(P组,n=6)，腹腔内注射戊巴比妥钠25 mg/kg麻醉后建立ECC心肌缺血再灌注模型。P组于ECC前静脉注射PDTC 20 mg/kg，C组静脉注射等量生理盐水。分别于ECC前、阻断主动脉60 min、开放主动脉30 min和60 min时，测定心肌线粒体超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶(GSH-PX）、丙二醛(MDA)、总抗氧化能力(T-AOC)和线粒体肿胀度(MSD)。结果 与ECC前比较，两组阻断主动脉后SOD、GSH-PX及T-AOC均降低(P＜0.01)，MDA和MSD含量均升高；开放主动脉后C组SOD、GSH-PX及T-AOC均降低(P＜0.01)，MSD和MDA含量均升高(P＜0.01)；P组在开放主动脉60 min时SOD、GSH-PX、T-AOC、MSD和MDA含量差异无统计学意义(P＞0.05)。P组在阻断主动脉60 min时和开放主动脉后SOD、GSH-PX及T-AOC均显著高于C组(P＜0.01)，MSD和MDA含量均显著低于C组(P＜0.01)。结论 ECC中使用PDTC可提高心肌线粒体抗氧化能力，减轻心肌线粒体损伤。 【关键词】 吡咯烷二硫氨基甲酸酯；线粒体；体外循环；心肌保护 Abstract: OBJECTIVE To observe the effect of pyrrolidine dithiocarbamate(PDTC)on canine myocardial mitochondrial injury during extracorporeal circulation (ECC). METHODS Twelve healthy mongrel dogs undergoing ECC were randomly allocated into control group(group C,n=6)and PDTC pretreatment group(group P,n=6).All dogs were anesthesitized with 2.5% sodium pentobarbitone 25 mg/kg.After tracheal intubation,the myocardial ischemia-reperfusion injury model during ECC was made by declamping(DC)after aortic cross-clamping(AC)for 60 min.In group P,PDTC 20 mg/kg was administered intravenously before ECC，while in group C physiological saline was given instead of PDTC.Myocardial mitochondria were isolated before ECC,60 min after AC，30 min and 60 min after DC for determination of superoxide dismutase (SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),total anti-oxidation capacity (T-AOC) and mitochondrial swelling degree(MSD). RESULTS The myocardial mitochondria of SOD,GSH-PX and T-AOC were decreased，while MDA and MSD were increased after AC as compared to the value before ECC in both groups(P＜0.01).SOD,GSH-PX and T-AOC were decreased，while MDA and MSD were increased after DC as compared to the value before ECC in group C(P＜0.01).The myocardial mitochondria of group P at 60 min after DC had not significant differences