复方参七汤对胃、结直肠癌抑制作用实验探究.docVIP

复方参七汤对胃、结直肠癌抑制作用实验探究 　 作者：钱晶晶，沈洪薰，陆\飞，周树军，陈玉泉 【摘要】 　　目的：研究复方参七汤治疗胃、结直肠癌的作用机制。方法：（1）复方参七汤对人胃癌细胞的体外抑制实验；（2）对MNNG诱发大鼠腺胃癌的体内抑制诱癌率实验；（3）对裸鼠结肠癌肝转移的体内抑制实验。结果：（1）复方参七汤对人胃癌BGC-823细胞株半数杀伤浓度（LC50）为0.048 g/ml，随药物浓度增高，杀伤作用亦增强；半数抑制浓度（IC50）为0.007 g/ml，随药物浓度增高，抑制有丝分裂指数逐渐减小，而抑制细胞集落形成亦增大；（2）复方参七汤对MNNG诱发大鼠腺胃癌发生率明显降低，实验组为6.9%(3/43)显著低于对照组58.9%(23/39)，（Plt;0.01)；对MNNG诱发大鼠腺胃癌组织中PCNA和CyclinD1阳性表达明显降低，实验组为13.88%(5/36)和22.86%(8/35)，显著低于对照组75.76%(25/33)和62.86%(22/35)，（Plt;0.01)；（3）复方参七汤对人结肠癌LS174t细胞株接种裸鼠肝转移的肝转移数、肝转移癌重和肝癌重/肝重明显降低，实验组为2.42±0.99，63.67±21.29和8.90±3.10，显著低于对照组4.17±1.99，94.32±37.86和13.20±5.30（Plt;0.05)；对裸鼠肝转移血中CK20 mRNA表达的相对值减少，实验组为0.398±0.143显著低于对照组0.518±0.090，（Plt;0.05)；对裸鼠肝转移组织中nm23H1-NDPK阳性表达呈过表达，实验组为66.67%(8/12)显著高于对照组16.6%(2/12)，（Plt;0.05)。结论：复方参七汤在体外实验有杀伤癌细胞作用；在体内实验抑制大鼠对MNNG诱癌率；抑制裸鼠对人结肠癌细胞肝转移的作用。 【关键词】 胃癌 复方参七汤 肝转移 　　[Abstract] Objective: To investigate the mechanism of therapeutic effect of compound Shenqitang on the gastric and colorectal carcinomas. Methods: Several experiments had been done, including:(1) the inhibitory effect of compound Shenqitang on human gastric adenocarcinoma cell line in vitro；(2) its inhibitory effects on Wistar rat gastric adenocarcinoma induced by MNNG; and (3) its inhibitory effect on the hepatic metastases of colorectal carcinoma in mice. Results: （1）Obvious inhibitory effects of compound Shenqitang had been found on the cell division and colony form of human gastric adenocarcinoma cell line BGC-823 in vitro. The LC50 was 0.007g crude drug/ml in colony form experiment. The drug had potent killing effect in higher concentration, with LC50 of 0.048g crude drug/ml；（2）The incidence of gastric adenocarcinoma in the experimental group(6.9%) was significantly lower than that in the control group(58.9%).The positive rates of PCNA and cyclin D1 in the experimental group were 13.88%(5/36) and 22.86%(8/35), which were significantly lower than those in the control group（Plt;0.01)；（3）Compound Shenqitang inhibited hepatic metastases of colorectal carcinom