小麦籽粒淀粉分支酶同工酶结构组成及时空表达-作物学报.PDFVIP

作物学报 ACTA AGRONOMICA SINICA 2014, 40(12): 21762182 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.02176 小麦籽粒淀粉分支酶同工酶结构组成及时空表达 刘正帅1 刘贵芬1 杨明煜2 贾 晓1 李运祥1 赵法茂1,* 1 , 271021; 2 , 271000 : , 8 , (Native)SDS (SDS)SBE , SBE 4 SBE , B SBEIIa , A D , D SBEIIa , B, A ; , B i i SBEIIa SBE , 86~92 kD SBE , DiB SBEIIa SBE , SBE , : ; ; ; ; Constitution and Spatiotemporal Expression of Starch Branching Enzyme in Developing Wheat Grain 1 1 2 1 1 1,* LIU Zheng-Shuai , LIU Gui-Fen , YANG Ming-Yu , JIA Xiao , LI Yun-Xiang , and ZHAO Fa-Mao 1 College of Biology and Enology, Taishan University, Tai’an 271021, China; 2 Tai’an No.1 Middle School, Tai’an 271000, China Abstract: This study aimed at disclosing the enzymatic mechanism in amylopectin synthesis in wheat (Triticum aestivum L.). The isozyme forms, organ localization, spatiotemporal expression profile and subunits constitution of starch branching enzyme (SBE) were identified in eight wheat cultivars from different provenances using native polyacrylamide gel electrophoresis (Native) and SDS. Four SBE isozymes were detected in wheat endosperm, in which isozymes B and SBEIIa were localized in en- dosperm and leaf, whereas isozymes A and Di were exclusively present in