斑马鱼视网膜感光细胞的发育及视蛋白的表达-中国眼耳鼻喉科杂志.PDF

84 ·研究生论文· 斑马鱼视网膜感光细胞的发育及 视蛋白的表达 张艳琼王跃祥’蒋谬’宋后燕’ 王文吉 【摘要】 目的研究斑马鱼视网膜感光细胞的发育和视蛋白的表达，明确利用斑马鱼研究视网膜和感光 细胞的可行性。方法制备斑马鱼视网膜感光细胞视蛋白(视杆细胞视紫质、视锥细胞紫外线视蛋白和视锥细 胞蓝色视蛋白)的RNA探针。收集受精后72，96，120h的斑马鱼眼球组织进行切片、电镜观察和整体原位杂 交。结果斑马鱼的神经视网膜分层排列，包括3个细胞层和2个丛状层。随时间发展，视网膜的发育逐渐成 熟，3个细胞层的细胞排列更加整齐，感光细胞的外节盘发育更加成熟，3种视蛋白的表达逐渐增强，范围扩大。 结论利用斑马鱼进行视网膜和感光细胞的研究是可行的，视蛋白可作为感光细胞的标记。(中国眼耳鼻喉科 杂志，2008，8：84-86) 【关键词】感光细胞；视蛋白；视网膜；斑马鱼 of of inthezebrafishZHANG Yue— retinal and Developmentphotoreceptorsexpressionopsin Yan—qiong，WANG EarNoseandThroatHos一 xiang‘．JlANGQiu‘．SONGHou·yan’，WANG耽n-ji．DepartnvmtofOphthalmology。Eye ∥tal，FndanUniversity，Shanghai200031，China author：ZHANG Corresponding Yah—qiong．Email：yanqiongzhan9401@yahoo．com．ca To the ofretinal and of intheze- 【Abstract】0bjectivestudydevelopmentphotoreceptomexpressionopsin for brafish．Tothe of zebrafishtO retinaand RNA ze- identifyfeasibilityusing stuay photoreceptors．Methodsprobes brafish rod wavesensitive shortwavesensitive opsinrhodopsin(Rho)，shortl(SWSl)and 2(sws2)were photoreceptor were h 120h larvae cdlected72 and prepared．Zebrafish h，96 post·fertilizationrespectively．Eyehistolo百calslice， whde in．situ were ItWagfoundthattheretina electmnicmicroscopeand mount sample hybridizationprepared．Results ofthezebrafishWas threecell andtwo time passed laminated，includinglayers plexiformlayers．As by，thearrangement ofthecell becamemoreandmore outer ofthe showedtOhefurthermat