the prognostic impact of tgf-β1, fascin, nf-κb and pkc-ζ expression in soft tissue sarcomastgf-β1对预后的影响,fascin nf-κb和pkc-ζ表达在软组织肉瘤.pdfVIP

The Prognostic Impact of TGF-b1, Fascin, NF-kB and PKC- f Expression in Soft Tissue Sarcomas 1,2 1,2 2 3,4 3 Andrej Valkov *, Sveinung W. Sorbye , Thomas K. Kilvaer , Tom Donnem , Eivind Smeland , Roy M. Bremnes3,4, Lill-Tove Busund1,2 1 Department of Clinical Pathology, University Hospital of Northern Norway, Tromsø, Norway, 2 Institute of Medical Biology, University of Tromsø, Tromsø, Norway, 3 Department of Oncology, University Hospital of Northern Norway, Tromsø, Norway, 4 Institute of Clinical Medicine, University of Tromsø, Tromsø, Norway Abstract Aims: Transforming growth factor- b (TGF-b), fascin, nuclear factor-kappa B (NF-kB) p105, protein-kinase C-zeta (PKC-f), partioning-defective protein-6 (Par-6), E-cadherin and vimentin are tumor promoting molecules through mechanisms involved in cell dedifferentiation. In soft tissue sarcomas, their expression profile is poorly defined and their significance is uncertain. We aimed to investigate the prognostic impact of TGF-b1, NF-kB p105, PKC-f, Par-6a, E-cadherin and vimentin in non-gastrointestinal stromal tumor soft tissue sarcomas (non-GIST STSs). Patients and Methods: Tumor samples and clinical data from 249 patients with non-GIST STS were obtained, and tissue microarrays (TMAs) were constructed for each specimen. Immunohistochemistry (IHC) was used to evaluate marker expression in tumor cells. Results: In univariate analysis, the expression levels of TGF-b1 (P = 0.016), fascin (P = 0.006), NF-kB p105 (P = 0.022) and PKC- f, (P = 0.042) were significant indicators for disease specific survival (DSS). In the multivariate an