氨基柱的使用和保养（Use and maintenance of amino column）.docVIP

氨基柱的使用和保养（Use and maintenance of amino column） Use and maintenance of amino column An amino column is a chromatographic column that can be used both in normal and antiphase conditions. But the normal solvent and the reverse solvent are immiscible, which cannot be ignored. For the newly purchased column, first of all, please pay attention to open the analysis and test manual and understand the preservation solvent of the column. If the solvent is different from the polarity of the mobile phase you are going to use, it will not dissolve. Please use isopropyl alcohol first. During the transition, attention is paid to the high viscosity of isopropanol, which leads to high column pressure and proper lowering of flow velocity. If there is buffer salt in the mobile phase, the transition of the same proportion of mobile phase without buffer salt should be used first to avoid the precipitation of buffer salt. Column effect test: One of the indicators I use to judge whether a user is experienced or not is whether users are accustomed to the preparation of standard column solutions. When it comes to column effect testing, I can provide you with a common method, and you can also use the same standard solution and mobile phase to detect silica gel column and cyano column. Mobile phase: 10% ethyl acetate + 90% hexane, flow rate 1 Standard solution: ethyl benzene (or toluene) + methyl benzoate Detection wavelength 254nm This method is particularly suitable for new post (new column is often preserved in the positive solvent) when testing, and if there is a problem, can communicate with suppliers in a timely manner. Also suitable for columns to be placed for a long period of time before the full cleaning test, as a record retention. In the ordinary course of use, if the analyte is fixed and repeated, we can certainly judge from the separation and analysis of the analyte, but if the analyte and the analytical conditions are not the same at the same time, Regular examination of column