Effect of triptolide on secretion of inflammatory cellular factors TNF—α and IL—8 in peritoneal macrophages of mice activated by lipopolysaccharide.doc

Effect of triptolide on secretion of inflammatory cellular factors TNF—α and IL—8 in peritoneal macrophages of mice activated by lipopolysaccharide BACKGROUND： Research has been carried out to look for safe and effective anti-inflammation drugs from traditional Chinese herbal medicine. As a powerful research technology of life science， molecular biology has entered many areas of traditional Chinese medicine.This study aimed to investigate the effect of triptolide on tumor necrosis factor-α （TNF-α） and interleukin-8 （IL-8） of peritoneal macrophages activated by lipopolysaccharide （LPS） in mice. METHODS： Peritoneal elicited macrophages were separated， purified and activated by LPS in mice， then cultured in vitro with triptolide at different concentrations. The activity of TNF-α and the level of IL-8 of cellular supernatants were determined by MTT colorimetric assay and ELISA， respectively. RESULTS： The activity of TNF-α in macrophages was significantly inhibited （P0.01） by triptolide （10-1-101?g/ml） during 4-24 hours in a time- and dose-dependent manner. The level of IL-8 in macrophages was significantly inhibited （P0.01） by triptolide （10-1-101?g/ml） in 12 hours in a dose-dependent manner. CONCLUSION： Triptolide could inhibit the activity of TNF-α and the level of IL-8 in macrophages activated by LPS. KEY WORDS： Triptolide； Lipopolysaccharide； Macrophage； Tumor necrosis factor-α； Interleukin-8 World J Emerg Med 2010；1（1）：70-74 INTRODUCTION Tripterygium wilfordii， one of the famous Chinese medical material， has been used to treat some autoimmune diseases such as dermatitis eczema. Many components have been extracted from Tripterygium wilfordii， with activities such as anti-inflammation， anti-fertility， anti-oncosis， antibiosis， immunosuppression， etc. Among them the extracted components include alkaloid， diterpenes， triterpenes， sesquiterpene， glycoside， etc.[1] Triptolide is one of the over 70 effective components extracted from Tripterygium