原创力文档Effects of bone marrow—derived mesenchymal stem cells engraftment on vascular endothelial cell growth factor in lung tissue and plasma at early stage of smoke inhalation injury.doc
- 1、本文档共18页，可阅读全部内容。
- 2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
- 3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
查看更多
Effects of bone marrow—derived mesenchymal stem cells engraftment on vascular endothelial cell growth factor in lung tissue and plasma at early stage of smoke inhalation injury
Effects of bone marrow—derived mesenchymal stem cells engraftment on vascular endothelial cell growth factor in lung tissue and plasma at early stage of smoke inhalation injury
BACKGROUND： This study was undertaken to determine the effect of mesenchymal stem cells （MSCs） engraftment on vascular endothelial cell growth factor （VEGF） in lung tissue， plasma and extravascular lung water at early stage of smoke inhalation injury.
METHODS： A rabbit smoke inhalation injury model was established using a home-made smoke inhalation injury generator， and rabbits were divided into two groups randomly： a control group （S group， n=32） and a MSCs treatment group （M group， n=32）. 10 ml PBS was injected via the ear marginal vein immediately at injury into the S group. Third generation MSCs with a concentration of 1×107/10 ml PBS were injected via the ear marginal vein immediately at injury into the M group. VEGF in peripheral blood and lung tissue were measured at 0 （baseline）， 2， 4 and 6 hours after injection respectively and analyzed. The right lungs of rabbits were taken to measure lung water mass fraction.
RESULTS： In the lung tissue， VEGF decreased gradually in the S group （P0.05） and significantly decreased in the M group （P0.05）， but it increased more significantly than the values at the corresponding time points （P0.05）. In peripheral blood， VEGF increased gradually in the S group （P0.05） and markedly increased in the M group （P0.05）， but it decreased more significantly than the values at corresponding time points （P0.05）.
CONCLUSION： MSCs engraftment to smoke inhalation injury could increase VEGF in lung tissue， decrease VEGF in plasma and reduce extravascular lung water， indicating its protective effect on smoke inhalation injury.
KEY WORDS： Mesenchymal stem cells； Smoke inhalation injury； Vascular endothelial cell growth factor； Extravascular lung water； Rabbit
World J Emerg Med 2010；1（3）：224-228
INTRODUCTION
Pulmonary injury from smoke inhalation i
文档评论（0）