醚菊酯检测方法（Ethofenprox detection method）.docVIP

醚菊酯检测方法（Ethofenprox detection method） Preparation of test solution A extraction method Cereals, legumes, and seeds After grinding the sample through 420 m standard mesh, weigh the 10.0g and add 10mL water for 2 hours. Add 100mL of acetone, stirring after 3 minutes, with a thick coating of 1cm diatomite filter, filtration in grinding mouth decompression concentrator. Remove the residue on the filter paper, adding acetone 50mL, stirring after 3 minutes, according to the same operation, with the filtrate in vacuum concentrator, concentration below 40 DEG C to about 30mL. Move it into pre injection 100mL 10% Sodium Chloride Solutions 300mL split funnel. Use the solanad type bottle 100mL hexane washing the vacuum concentrator, with washing liquid in a separatory funnel. With oscillator vibration, 5 minutes after the stationary, n-hexane layer into the 300mL triangle bottle. A 50mL hexane is added to the water layer, and the n-hexane layer is combined in the triangle bottle according to the same operation as described above. Adding proper amount of anhydrous sodium sulfate, from time to time, oscillation, mixing, placing 15 minutes, filter into the grinding mouth decompression concentrator. Then the 20mL hexane washing flask, the residue washing solutions on filter paper, repeated two times, with two washing liquid in vacuum concentrator, 40 DEG C to remove hexane. 30mL hexane was added into the residue and moved into the 100mL separating funnel. Add 30mL n-hexane saturated with acetonitrile, the oscillator oscillation after 5 minutes of intense, static, acetonitrile layer into the grinding mouth decompression concentrator. The hexane layer was added 30mL n-hexane saturated acetonitrile, according to the same operation two times, with acetonitrile layer on the vacuum concentrator, 40 DEG C to remove acetonitrile. Residue was dissolved by adding 5mL hexane. Fruits, vegetables, tea and hops Fruits and vegetables: take about 1kg samples accurately. If necessary, add a proper