野菊花中绿原酸提取工艺的研究（Study on the extraction process of chlorogenic acid in the wild chrysanthemum）.docVIP

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野菊花中绿原酸提取工艺的研究（Study on the extraction process of chlorogenic acid in the wild chrysanthemum）.doc

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野菊花中绿原酸提取工艺的研究（Study on the extraction process of chlorogenic acid in the wild chrysanthemum）

野菊花中绿原酸提取工艺的研究（Study on the extraction process of chlorogenic acid in the wild chrysanthemum） Keywords wild chrysanthemum flower; chlorogenic acid; orthogonal test; spectrophotometry; extraction process; Abstract:ObjectiveTo obtain optimum extraction technology of chlorogenic acid from Flos Chrysanthemi Indici.MethodsThe extraction processes for chlorogenic acid from Flos Chrysanthemi Indici. with water bath extraction and ultrasonic wave were investigated using the content of chlorogenic acid as index. The optimal extraction conditions were determined with orthogonal design. ResultsFor water bath extraction, F. Chrysanthemi Indici was extracted with 12 times the mass of 70% ethanol and refluxed at 80 min. The content 40 C for of flavonoids was 0.35%. For ultrasonic wave extraction, F. Chrysanthemi Indici was extracted with 15 times the mass of 60% ethanol perimpregnating for 40 min and extracted for min. The content of flavonoids w 45 As 0.35,% ConclusionThe, processing, method, is, feasible Key, words:F., Chrysanthemi, Indici.; Chlorogenic acid; Orthogonal design; Spectrophotometry; technology; Extracting Chrysanthemum (Flos Chrysanthemi Indici) also known as wild Huang Ju, bitter Yi, Compositae chrysanthemum Chrysanthemum indicum L. dried flower heads. Its flavor, bitter, pungent, cold, liver, heart meridian. It has the function of clearing away heat and toxic materials. Commonly used in furuncle carbuncle swollen, swollen red eyes, headache and dizziness and other symptoms 1. Chlorogenic acid is one of the main active components in wild chrysanthemum. It is reported that chlorogenic acid has antibacterial, antiviral, anti lipid peroxidation and many other pharmacological activities, and has a significant effect on enhancing gastrointestinal peristalsis and promoting gastric secretion (2). Considering that chlorogenic acid has many physiological activities, it is important to study chlorogenic acid in Chrysanthemum indicum. In order to effectively extract chlorog