HPLC 测定银杏黄酮苷元固体分散体胶囊剂的含量.PDFVIP

·药物分析与检验 · HPLC 测定银杏黄酮苷元固体分散体胶囊剂的含量 钱一鑫，康冀川* ，何珺(贵州大学西南药用生物资源教育部工程研究中心，贵阳 550025) 摘要：目的 建立高效液相色谱法(HPLC)测定银杏黄酮苷元固体分散体胶囊中有效成分的含量。方法 以 Hypersil ODS (4.6 mm×150 mm ，5 μm) 为色谱柱；甲醇-4‰醋酸水溶液(48 ∶52)为流动相；流速为 0.8 mL·min1 ，检测波长 360 nm ，柱 温 45 ℃。结果 槲皮素和山奈素 5~100 μg·mL1 浓度内线性关系良好，异鼠李素在 3.4~68.0 μg·mL1 浓度内线性关系良 好，平均加样回收率分别为 99.31% ，99.35%和 98.18% ，RSD 分别为 2.12% ，2.28%和 1.65%(n=6) ，精密度良好。结论 本 方法灵敏、准确、专属性强，可作为该制剂的含量测定方法。 关键词：银杏黄酮苷元固体分散体胶囊；HPLC ；含量测定 中图分类号：R284.1 文献标志码：B 文章编号：1007-7693(2010)08-0723-04 Determination of the Ginkgo Flavonoid Aglycon Solid Dispersions Capsules by HPLC * QIAN Yixin, KANG Jichuan , HE Jun(The Engineering and Research Center for Southwest Bio-Pharmaceutical resources of National Education Ministry of China, Guizhou University, Guiyang 550025, China) ABSTRACT: OBJECTIVE A HPLC method was established to determine the content of ginkgo flavonoid aglycon solid dispersions capsules. METHODS Chromatographic condition included the Hypersil ODS column (4.6 mm×150 mm, 5 μm) and mobile phase consisting of methanol - 4‰ acetic acid solution(48 ∶52). The flow rate was 0.8 mL·min1, the detection wavelength was set at 360 nm, the column temperature was 45 ℃ . RESULTS The linear calibration curves were obtained in the concentration range of 5.0100.0 μg·mL1 and 3.4068.0 μg·mL1. The mean recovery was 99.31%, 99.35% and 98.18%, RSD was 2.12%, 2.28% and 1.65%(n=6) with good precision. CONCLUSION The method is sensitive, accurate and high selectivity. A satisfactory result to determine the dissolution of this dosage form was obtained with this method. KEY WORDS ：Ginkgo flavonoid aglycon solid dispersions capsules; HPLC; content determination 黄酮类化合物是银杏叶中的主要药效成分，银 用率上起着关键性作用，一般认为苷类不通过小肠 杏叶黄酮具有广泛的生物活性，包括抗氧化活性、 壁，其在肠的蠕动下，行进的过程中，通过小肠和 抗肿瘤活性、抗炎症和免疫调节细胞效应等[1] 。银 结肠中细