石榴皮多糖硒酸酯制备工艺参数优化及其结构分析-食品科学.pdfVIP

40 2016, Vol.37, No.10 食品科学 ※工艺技术 石榴皮多糖硒酸酯制备工艺参数优化及其 结构分析 1 2 1 1 1 1 王占一 ，戴 博 ，张立华，王玉海，郑丹丹，王 飞 （1.枣庄学院生命科学学院，山东 枣庄 277160 ；2. 中国人民解放军空军总医院药学部，北京 100142 ） 摘 要：以石榴皮多糖与亚硒酸钠为原料制备石榴皮多糖硒酸酯，以产物硒含量和收率为指标，原料配比、硝酸体积 分数、反应温度和反应时间为单因素，通过单因素试验和Box-Behnken试验设计方法对工艺条件进行优化，并对产物 结构进行分析。结果显示：石榴皮多糖硒酸酯最佳制备工艺条件为原料配比1∶1.0 （g/g ）、硝酸体积分数0.61%、反应 温度77.0 ℃、反应时间11.3 h，在该工艺条件下，制得石榴皮多糖硒酸酯中硒含量为4.48 mg/g ，产物收率为41.87%； 紫外光谱、红外光谱和热重分析充分证实石榴皮多糖硒酸酯中含有Se＝O键、Se—O键和Se—C键。优化后的石榴皮多 糖硒酸酯制备工艺条件实现了石榴皮多糖的硒化，为石榴皮资源的充分开发利用提供良好条件。 关键词：石榴皮多糖；亚硒酸钠；多糖硒酸酯；紫外光谱；红外光谱；热重分析  Optimized Preparation and Structural Analysis of Polysaccharide Selenate from Pomegranate Rind 1 2 1 1 1 1 WANG Zhanyi , DAI Bo , ZHANG Lihua , WANG Yuhai , ZHENG Dandan , WANG Fei (1. College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China; 2. Department of Pharmacology, General Hospital of Air Force, People’s Liberation Army of China, Beijing 100142, China) Abstract: In this paper, polysaccharide selenate was prepared via the reaction between pomegranate rind polysaccharide and sodium selenite. Four reaction conditions including ratio between the substrates, final HNO3 concentration, reaction temperature and time were optimized based on the selenium (Se) content and yield of product using single factor experiments and Box-Behnken experimental design. At the same time, the structure of the obtained product was analyzed. Results showed that the optimum reaction conditions for preparing polysaccharide selenate were established as follows: ratio betw