副溶血弧菌对南美自对虾生理生化指标的影晌-上海海洋大学学报.pdfVIP

第 16 卷第2 期 上海水产大学学报 Vol. 16 , No.2 2007 年3 月 JOURNAL OF SHANGHAI FISHERIES UNIVERSITY Mar. ,2007 文章编号: 1004 -7271(2007)02 -0162 -07 副溶血弧菌对南美自对虾生理生化指标的影晌 霍秀梅1 ， 2 王斌毛连菊郭郁桂远明l (1.大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室，辽宁大连 116023 ; 2. 黑龙江生物科技职业学院水产系，黑龙江哈尔滨 150025) 摘 要:在自然条件下从患红体病的养殖南美白对虾肝脏内分离出病原菌一一副溶血弧菌，将该茵通过肌肉 注射的方式，对南美白对虾做感染实验，以探讨副溶血弧菌对南美白对虾生理生化指标的影响。注射弧菌后 南美白对虾死亡率与时间、浓度呈正相关，与半致死剂量呈负相关;虾体肌肉、肝脏组织的超氧化物歧化酶 (SOD) 、溶菌酶(LSZ) 酶活性随弧菌浓度的增加呈下降的趋势，而过氧化物酶(POD) 酶活性呈上升趋势，这可 能是应激反应;醋酶同工酶(EST) 酶带表现出缺失，苹果酸脱氢酶同工酶(MDH) 具有明显的组织特异性，并 且两种酶在肌肉组织中表达最强。副溶菌弧菌感染后破坏了虾体的免疫系统，导致免疫机能的损坏，防病、抗 病能力下降，进一步导致虾体患病，甚至死亡。 关键词:副溶血弧菌;南美白对虾;生理生化指标 中图分类号:S 945. 1 文献标识码:A The effects of physiological and biochemical index for Penaeus vannmei infected with Vibrio parahaemolyticus ZHAI Xiu-mei 1,2 , WANG Bin 1 , MAO Lian-ju 1 , GUO Yu 1 , GUI Yuan-ming 1 (υ 1. Key μL且bωoraαtωorηyofMαr巾i比Cα础￡u Dαliαn Fz臼￥herie臼s Uni口四ver.ηsity ， Dαliαn 116023 ,Chinα ; 2. Depart阳nt ofAquaculture , Heilongjiαng Vocational College of Biology Science and Technology , Hα， rbin 1500万， China) Abstract :The effects of physiological and biochemical index for white-leg shrimps ( Penαe山 vαnnmei) infected with 町brio pαrahαemolyticus were studied in this paper. It is a kind of bacteria -民brio pαrahαemolytic时 that was isolated from hepatopancreas of diseased shrimps farmed under natural conditions. The infection experiment that healthy shrimp muscles were injected with the isolated bacteria - Vibrio pαrahαemolyticus was carried