牛奶中蛋白质检测研究.pdf

毕业设计（论文）题目： 奶制品中蛋白质检测方法学研究 学 院： 生命学院 专 业： 生物技术 班 级： 班 姓 名： 刘 彤 指导教师： 王鲁娜 北京理工大学本科生毕业论文 摘 要 本文从物理、化学和生物学方面，概括了近十年牛奶掺假的国内外检测技术。 并以ELISA 技术为基础，对样品离心去除脂质层，优化抗体浓度、反应时间、 温度等实验条件，设计同时检测酪蛋白与乳球蛋白混合样品的实验方法。评价实 验的精确度和准确性。加入三聚氰胺、三聚氰酸、尿素和豆粉几种常用掺假物后， 仍能准确测得奶制品中酪蛋白与 β-乳球蛋白的含量。结果表明，ELISA 技术检 测双蛋白的板间误差为 2.9 % ；回收率为97.98 % ± 6.4% ，优于文献中其他方法 10%左右的检测精密度和准确性。三聚氰胺、三聚氰酸、尿素和豆粉所造成的最 大检测误差小于 15%，符合生物样本分析要求。因此，该检测技术具有精度高， 数据可靠且抗干扰能力强的优点。其应用为奶样中全蛋白的检测提供了新的方 法。 关键词：奶制品；酪蛋白；β-乳球蛋白；ELISA Abstract This article first made a summary of the last decade of milk adulteration detection technology at home and abroad through aspects of physics , chemistry and biology. Then, based on ELISA technique , removed the lipid layer by centrifugal effect, optimized experimental conditions of consistence of antibody, reacting time, temperature etc. D esigned an experiment to testing casein and lactoglobulin at the same time. Evaluated its precisi on and accuracy. Added melamine, cyanuric acid, urea and soybean meal to test the specific of the technology through interference. The results show that the cubicle error of dual protein through ELISA detection was 2.9 % and the rate of recovery was 97.98 % ± 6.4%. These results were better than others detections which precision were about 10 %. The max error coursed by I 北京理工大学本科生毕业论文 melamine, cyanuric acid, urea and soybean meal was less than 15 % which was in line with the requirements of analysis of biological samples . Thus, this detection te