胎儿皮肤中fgfr2表达的免疫组织化学研究.doc

胎儿皮肤中FGFR2表达的免疫组织化学研究 伊海英1 孙晓艳2 付小兵2 任明姬1 张广田12 （1内蒙古医学院，内蒙古呼和浩特 010059；2 解放军总医院，北京 100） 要目的 免疫组织化学方法研究不同发育时期胎儿皮肤中FGFR2的表达及分布，探索FGFR2在皮肤发生、发育中的作用，为研究皮肤创伤修复与再生提供实验参考。方法 不同发育时期胎儿皮肤，取材、固定、制成石蜡切片，SP法检测FGFR2的表达。结果 胚胎发育期FGFR2在表皮细胞表达较弱，甚至呈阴性表达。表皮分层期时，表皮层开始增厚，并可见毛囊的初级原基；FGFR2在表皮层和毛囊初级原基内呈弱阳性表达。毛囊形成期时，毛囊的结构初步形成，体积细小，形态幼稚；FGFR2主要在毛母质细胞内表达并且随着胎龄增加，表达范围逐渐扩大，表达量逐渐增多。毛囊发育成熟期后，毛囊的结构发育相对成熟，FGFR2则在表皮层、毛细血管内皮细胞及皮脂腺的导管部均有表达，尤其在毛母质细胞呈强阳性表达。结论 FGFR2在胎儿皮肤中的表达与分布与毛囊的发生发育密切相关。FGFR2的表达上调能够促进毛囊干细胞的增殖，并诱导其定向分化形成终末组织功能细胞。 关键词胚胎发育；FGFR2；免疫组织化学；再生 基金项目国家重点基础研究发展计划资助项目(2005CB522603) Supported by National Basic Research Program of China (2005CB522603) 作者简介：伊海英（1981-），女，内蒙古通辽人，在读硕士，主要从事皮肤的创伤修复与再生方面的研究。电话：(010)E-mail：xiao_yying@163.com △通讯作者：uxb@ Immunohistochemical Studies on the Distribution and Expression of FGFR2 in Human Fetal Skin YI Hai-ying1, SUN Xiao-yan2, FU Xiao-bing2, REN Ming-ji1, ZHANHG Guang-tian1, LIU Hui-ling2?(1Department of Histology and Embryology, Inner Mongolian Medical College, Hohhot, 010059, China；2Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing 100853, China) Abstract: Objective To investigate the expression and distribution characteristics of FGFR2 in the human fetal skin during various stages of the gestation, and to explore its biological significance in the cutaneous development and wound healing process. Methods The expression of FGFR2 in the fetal skin was analyzed by using immunohistochemical method. Results Positive immunohistochemical signals of FGFR2 could be found in fetal skins, which were closely related to the developmental patterns of hair follicles. The expression of FGFR2 was first detected in the cellular membrane of epidermal cells in the undifferentiated epidermis during the embryonic period. Coinciding with the stratification of the epidermis (the period of epidermal stratification), FGFR2 was observed in the newly formed epidermal layers and primary hair germs. During the period of follicular keratinization, the