家兔动脉血压的神经体液调节（Neurohumoral regulation of arterial blood pressure in rabbits）.docVIP

家兔动脉血压的神经体液调节（Neurohumoral regulation of arterial blood pressure in rabbits） Neurohumoral regulation of arterial blood pressure in rabbits [purpose of the experiment] 1. to study the acute experimental method of directly measuring and recording the arterial blood pressure in rabbits 2. observe the effects of certain nerve and humoral factors on cardiovascular activity [experimental materials] rabbit Equipment: operating table, hemostatic forceps, eye scissors, BL420E+ biological function analysis system, trachea cannula, artery sleeve, artery clip, protective electrode, illuminating lamp, gauze, cotton ball, silk thread, syringe Reagent: saline, heparin, ethylurethanm (anesthetic), epinephrine, acetylcholine [experimental steps] 1. operation (1) anesthesia was taken with one rabbit, weighed and injected intravenously into the ear vein (1g/kg) for anesthesia. Anesthesia process should be slow, when the animal cornea unresponsive, pinch its thigh no response, you can stop the injection, to avoid excessive anesthesia to death. (2) the animal back cross fixed, will lower the neck hair cut. (3) by Adams apple margin do about 5-7cm long skin incision along the midline of the neck, subcutaneous connective tissue exposed to blunt separation, pipe threading, a transverse incision in the trachea with a scalpel, insert the endotracheal intubation, ligation. (4) the separation of cervical nerve blood vessel: separate sternohyoid and sternothyroid muscle and connective tissue in the trachea has a close, thin, thick walled blood vessels, is the aorta (can be seen inside the flow pulsating). There were two thicker nerves associated with the aorta, the largest being the vagus nerve, and the second sympathetic nerve. There was a very thin nerve between the two, and the depressor nerve. But the location of the depressor nerve is not fixed, and the two small nerves near the thicker nerve may be the depressor nerve, which can be stimulated and tested. After the vagus nerve and the depr