毛细管柱选择规则.txtaaa（Capillary column selection rule.Txtaaa）.docVIP

毛细管柱选择规则.txtaaa（Capillary column selection rule.Txtaaa） In order to select a suitable commercial capillary column, the following factors should be considered: fixed phase, internal diameter, column length and film thickness. The following are discussed in terms of these factors. 1, stationary phase In chromatography theory, the choice of fixed phase follows the principle of similarity, that is, the use of nonpolar stationary phases to analyze nonpolar substances Polar stationary phase analysis of polar substances, aromatic compounds fixed phase analysis of aromatic compounds. In the era of packed columns, this The idea is very correct, because at that time, separation is the first key factor to grasp, and there is no way out Think other. The reason for this is the inference from the plate theory that the greater the solubility of the component in the stationary phase, the same dissolution The greater the degree of separation, the greater the degree of divergence. However, in the capillary chromatography era, separation is no longer the first choice for analysis. The capillary has hundreds of thousands of blocks of trays, such as The large column is so efficient that it is no longer necessary to consider the separation problem adequately. This time, sample compatibility, use temperature, Stability, column loss, etc., may be the main reason for choice. For example, analysis of methanol, ethanol, propanol and other low-grade alcohols, Normal selection Class wax high polarity chromatographic column. However, in the case of less impurities, the choice of A non-polar column such as DB-1 It has higher flexibility and service life. The specific choice is based on the following rules: 1) because non polar capillary column has obvious stability, high use temperature and good chromatographic peaks, and so on A column with a small polarity should be used for the separation of the material. 2) analysis of hydrogen bonded substances using hydrogen bonded type PEG columns are bett