聚丙烯酰胺凝胶及蛋白质银染原理.pptVIP

聚丙烯酰胺凝胶的蛋白质银染原理 主讲人：杨群峰 2009-05-21 1. Introduction 1. Introduction The sensitivity was reported to be at least 100 times better than for Coomassie Brilliant Blue. Silver staining is a complex, multistep process, and many variables can influence the result. High-purity reagents and precise timing are necessary for reproducible, high-quality results. 2. Ag staining procedures a. Fixing: Treat with acid, this renders the macromolecules in the gel insoluble and prevents them from diffusing out of the gel during staining steps. Substances in the gel that interfere with silver staining, such as bu