多裂骆驼蓬提取物对玉米幼苗生长和细胞保护酶系的影响-植物研究.PDF

25　 2　　　　　　　　　　　　　　　　　　　　　　 2005　4 Vol. 25　No. 2　　　　　　　　　　　　BULLETIN OF BOTAN ICAL RESEARCH Apri,l 　2005 ` 刘建新　王毅民　赵国林 (, 　745000) 　　以不同浓度的多裂骆驼蓬提取液浸种处理玉米,研究对幼苗生长和细胞保 酶系的影 响。结果表明, 多裂骆驼蓬提取液浸种处理种子的萌发和种子中 α-淀粉酶活性受到明显抑制,抑 制作用随处理浓度提高而增强, 随培养时间延长而减弱。随着培养时间的延长, 浸种处理可显著 提高幼苗根系活力和叶片硝酸还原酶活性,促进植株生长,根和茎叶生长量增加,根冠比增大。多 裂骆驼蓬提取液浸种后显著降低叶片过氧化氢酶(CAT )、抗坏血酸氧化酶(ACO )活性,提高过氧 化物酶(POD )活性,促进根系和叶片过氧化物同工酶谱的数量表达。 　玉米;多裂骆驼蓬;根系活力;保 酶系;过氧化物同工酶 E ffect ofP eganum m ultisectum on seedling grow th and cell protective enzym e sy stem in m aize LIU Jian-Xin　WANG Y-iM in　ZHAO Guo-Lin (Departm ent of Life Science, Longdong University, Q ingyang　745000) Ab stract　M aize seedsw ere soaked in the solution of extracts ofP egan um m u ltisectum M axmi Bobr to study thew effect on seedling growth and cell protective enzyme system. The results show ed that the ge-r m ination ofm aize seeds and activity of α-amylase w ere suppressed apparently by treatment andw ith in- creasing this in the concentration of extracts the cultu ral time sharply increased or prolonged. W ith the tmi e of culture prolonged, the vigor of roots and activity of n itrate reductase in leavesw ere raised, impel the grow th of plantw ith the dry matterw eight of roots and shoot increasing and the ratio of root to shoot rising. The activity of catalase(CAT)and ascorbic acid oxidase(ACO )in leavesw ere reduced appa-r ently, the activity of peroxidase(POD )was increased, impelled the quantity expression of isoperoxidase patterns in root and leaf. K ey w ord s　maize;P egan um m u ltisectum ;vigor of roots;protective enzyme;isoperoxidase (P eg anum harm a la L. ) 、、、 , 、。 6 ,、