.与中国西北部栽培黄芪共生的优势根瘤菌为中慢生根瘤菌属.pdf

Systematic and Applied Microbiology 39 (2016) 141–149 Contents lists available at ScienceDirect Systematic and Applied Microbiology j o u r na l h o m e pa ge: w w w. elsev ier.de/syapm Genetic diversity and distribution of rhizobia associated with the medicinal legumes Astragalus spp. and Hedysarum polybotrys in agricultural soils Hui Yan a,b , Zhao Jun Ji a,b , Yin Shan Jiao a,b , En Tao Wang a,b,c , Wen Feng Chen a,b,∗ , Bao Lin Guo d , Wen Xin Chen a,b a State Key Laboratory of Agrobiotechnology, Beijing 100193, China b College of Biological Sciences and Rhizobia Research Center, China Agricultural University, Beijing 100193, China c Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México D.F., Mexico d Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China a r t i c l e i n f o a b s t r a c t Article history: With the increasing cultivation of medicinal legumes in agricultural fields, the rhizobia associated with Received 20 December 2015 these plants are facing new stresses, mainly from fertilization and irrigation. In this study, investi- Received in revised form 28 January 2016 gations on the nodulation of three cultivated medicinal legumes, Astragalus mongholicus, Astragalus Accepted 29 January 2016 membranaceus and Hedysarum polybotrys were performed. Bacterial isolates from root nodules of these legumes were subjected to genetic diversity and multi