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                .烟草赤星病菌拮抗性大豆根瘤内生菌的筛选及抑制作用_赵龙飞
                    
 本文摘自赤峰学院学报 
 
      * 
        
(/ ,    476000) 
                                                                                             
                                                                                             
16S rDNA  
 42% 7   
                                                                                             
                                                                                             
                                                                                             
                                                                                             
 
16S rDNA  
Screening and inhibition of antagonistic endophytic bacteria isolated from soybean (Glycine 
                                                          * 
max)   nodules   against  Alternarialongipes. ZHAO   Long-fei  ,   XU   Ya-jun,   HOU   Yi-ting,   ZOU 
Yan-hui,  LI  Ya-nan,  YANG   Zhi-hua,  LI  Xiao-yu,  ZHANG   Meng-yao(Henan   Province  Key 
Laboratory    of  Plant-Microbe   Interactions/College  of Life Sciences, Shangqiu   Normal 
University, Shangqiu 476000, Henan, China) 
Abstract:  Using Alternaria   longipes   as   tested   phytopathogen,   endophytic   bacteria   isolated   from 
soybean nodules were selected to study antagonistic effect by confrontation and metabolic liquid 
culture   methods. The   inhibited   hyphae   were  determined   by  microscopic   observation,  and  the 
screened strains were characterized by cell culture, physiological and biochemical characteristics 
tests,   16S   rDNA   sequencing,   phylogenetic   analysis   and   inoculation   trials   in   greenhouse.   The 
results indicated that seven of the endophytes exerted more than 42% inhibitory effect after the 
first  and  the  second  screening.  These  strains  belonged  to  genus  Bacillus,  Pseudomonas, 
Sinorhizobium   and Stenotrophomonas,   respectively.  Microscopic  observation showed  that  the 
affected   hyphae   ends   of A. 
                
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