白僵菌耐干旱孢子液体培养条件研究.docVIP

白僵菌耐干旱孢子的液体培养条件研究 Drought-resistant spores of Beauveria bassiana Liquid Culture Conditions 摘 要 白僵菌是一类以昆虫为寄主的具有好气性的病原真菌。 白僵菌在真菌分类学上属于半知菌，丝孢纲、丝孢目、丝孢科，白僵菌属，通常根据孢子的形态分为球孢白僵[Beauveria bassiana (Bais. ) Vuill . ]和卵孢白僵菌Beauveria tenell (Del . ) Siem. ，其中球孢白僵菌作为真菌杀虫剂已经被广泛研究和应用于生物防治农林果蔬等多种作物害虫，是真菌杀虫剂应用最多的一个种类。 白僵菌在持续长久控制害虫，保存多种生物种类，维护生态平衡方面具有不可替代的作用，它对人畜无毒、无害、无副作用，不致畸、不致癌、不污染环境，是一类比较理想的绿色环保型生物杀虫剂。 本文以白僵菌SZ21菌株为实验材料，通过配制不同种类和浓度的碳源，氮源，无机盐组合的液体培养基(NH4 )2SO4则是较理想的氮源。吐温80对白僵菌液生分生孢子的形成具有显著的影响，培养基中吐温80含量在0.4%～0.8%之间时,有利于液生分生孢子的形成，产孢量(n)达7.30×1010～24.13×1010 L– 1。 关键词：白僵菌；分生孢子；液体培养 Abstract Beauveria bassiana is a kind of insect host with a good air of pathogenic fungi. Fungus Beauveria bassiana in the taxonomy of imperfect fungi belonging to sub-door, Trichosporon classes, Trichosporon head, Trichosporon Branch, Beauveria bassiana is usually divided according to the form of spores of Beauveria white cadaver [Beauveria bassiana (Bais.) Vuill.] and egg bassiana Beauveria tenell (Del.) Siem., as the fungus Beauveria bassiana which pesticides have been widely studied and used in a variety of fruits and vegetables such as agriculture, forestry biological control of crop pests, is to kill fungus one of the largest pesticide application category. Beauveria bassiana in the continuing long-term control of pests, save a variety of species and maintaining ecological balance has an irreplaceable role, its human and animal non-toxic, harmless and without side effects, do not teratogenic, not carcinogenic, do not pollute the environment, is a kind of ideal green environmental protection of biological pesticides. In this paper, a strain of Beauveria bassiana SZ21 experimental materials, the preparation of different types and concentrations of carbon, nitrogen, inorganic salts liquid medium combination, dubbed the 4 × 106 spores / mL of the spore suspension; take a few 250mL flask fitted take on different liquid media, respectively, each 75 mL, 1mL spore suspension inoculated bottle; 300 rpm,