新城疫病毒VGGA毒株反向遗传系统的建立及最佳外源基因表达位置的确定.pdf

  VG/GA Newcastle disease, ND(Newcastle disease virus, NDV) NDV , NDV NDV 90% NDV NDV NDV NDV NDV VG/GA NDV NDV VG/GA NDV NDV NDV VG/GA RT-PCR VG/GA cDNA T7 MVA/T7Hep-2 cDNA ,NDV rVG/GA HAHI rVG/GA EID50TCID50MDTICPI rVG/GA VG/GA NDV VG/GA NDV VG/GA NDV VG/GA GFP NDV VG/GA NP P P M M F F HN HN L GFP EID50 TCID50 MDT ICPI   rVG/GA-GFP-NP/P rVG/GA-GFP-P/M rVG/GA-GFP-M/F rVG/GA-GFP-F/HN rVG/GA-GFP-HN/L rVG/GA rVG/GA GFP GFP 3` Real-time PCR GFP NDV mRNA 3` 5` GFP NDV VG/GA P M NDV mRNA NDV VG/GA P M :VG/GA   ESTABLISHMENT OF A REVERSE GENETICS SYSTEM FOR NEWCASTLE DISEASE VIRUS VG/GA STRAIN AND THE EVALUATION OF ITS OPTIMAL INSERTION SITE FOR FOREIGN GENES EXPRESSION ABSTRACT Newcastle disease virus (NDV) is the causative agent of Newcastle disease (ND), one of the most important poultry diseases worldwide, affecting a wide variety of birds and causing significant economic losses to the poultry industry. NDV strains have been classified into lentogenic, mesogenic or velogenic pathotypes. Velogenic strains are further classified into viscerotropic velogenic and neurotropic velogenic strains. Viscerotropic velogenic strains produce lethal hemorrhagic lesions in the digestive tract wit