响应面法优化小球藻吸附镉的条件-兰州大学.pdfVIP

兰州大学学报: 自然科学版, 2017, 53(4) / 8 月 Journal of Lanzhou University ：Natural Sciences，2017 ，53(4) / August 响应面法优化小球藻吸附镉的条件 葸玉琴, 任春燕, 朱巧巧, 杨 红, 孙对兄 西北师范大学 生命科学学院, 兰州 730070 摘 要：以吸附率和生物量为指标, 在考察单因素实验基础上, 利用响应面法确定小球藻吸附Cd2+ 的 最优条件. 通过分析得到: 各因素对小球藻吸附Cd2+ 均有显著性影响, 影响顺序为c(Cd2+)pH培养 温度. 用回归方程预测小球藻吸附Cd2+ 的最优条件为: 温度29.14 ℃、pH=6.67、c(Cd2+ )=30.74 μmol/ L, 小球藻的生物量和吸附率分别达到0.71 g/L、85.2%. 关键词：小球藻; 镉胁迫; 生物量; 吸附率; 响应面法 中图分类号：Q914.82, Q945.78 文献标识码：A 文章编号: 0455-2059(2017)04-0501-07 DOI: 10.13885/j.issn.0455-2059.2017.04.010 Optimized conditions of Chlorella vulgaris adsorbing cadmium by the response surface methodology Xi Yu-qin, Ren Chun-yan, Zhu Qiao-qiao, Yang Hong, Sun Dui-xiong College of Life Sciences, Northwest Normal University, Lanzhou 730070, China Abstract: With the adsorption rate and biomass as indicators and on the basis of single factors, the opti- mal conditions of Chlorella vulgaris adsorbing Cd2 + were determined using the response surface method, with the result that various factors on the adsorption of Cd2 + were significantly affected and the order of influence of the concentration being cadmiumsolution pHculture temperature. Using the regression equation to predict the optimal conditions for the adsorption of Cd2+ , the biomass and chlorella adsorption rate reached 0.71 g/L and 85.2% when the temperature, pH and the concentration of the culture fluid were maintained at 29.14 ℃, 6.67 and 30.74 μmol/L, respectively. Key words: Chlorella vulgaris; chromium stress; biomass; adsorption rate; response surface methodology [3] 近年来, 大量的矿产和电镀等工业废水排入 的潜在危害 . 藻类因具有吸附重金属的功能, 常 地面水或渗入地下水引起的重金属Cd 污染日益 被用来监测水体重金属的污染程度. 微藻对多种