大枣中氨基酸和微量元素的含量测定中国中药杂志.DOC

大枣中氨基酸和微量元素的含量测定 郑丽英 依明·尕哈甫 殷其蕾 贺锐锐 詹先王 王耀欣 何希辉 陈鑫 范田园* 韩南银 (北京大学药学院 中国北京 100191) 摘 要：目的 研究大枣中各种氨基酸和微量元素的含量测定。方法 采用氨基酸分析仪测定氨基酸的含量，电感耦合等离子体-质谱联用测定大枣多种微量元素的含量。结果 测得22种氨基酸，39种微量元素含量，其中天冬氨酸，谷氨酸含量在800(g/g以上，脯氨酸含量高达6333(g/g，重金属元素含量均低于《中国药典》(2005年版) 以及国家无公害蔬菜等各种限量标准。结论 大枣中含有丰富的氨基酸及微量元素，为进一步开发研究大枣提供了有用的参考。 关键词：氨基酸；微量元素；电感耦合等离子体-质谱 中图分类号：R284.1 文献标识码：A 文章编号： Determination of amino acids and trace elements about Jujube Zheng Li-ying, Yiming.Gaghafu, Yin Qi-lei, He Rui-rui, Zhan xian-wang, Wang Yao-xin, He Xi-hui, Chen Xin, Fan Tian-yuan *, Nanyin Han (School of Pharmaceutical Sciences, Peking University, Beijing P.R.China 100191) Abstract : Objective: To determine the contents of jujubes on amino acids and trace elements. Methods: Amino acid automatic analyzer was used to measure the contents of amino acids. As for trace elements in water extraction, inductively coupled plasma mass spectrometry detection(ICP-MS) and inductively coupled plasma atomic emission spectrometer (ICP-OES) were adopted to determine the precise level. All jujube simples came from Lvliang, Shanxi province. Results: The simple jujubes contained 22 amino acids and 39 kinds of trace elements. The contents of spartic acid, glutamic acid were above 800mg/g, and proline acid was up to 6333mg/g. besides. Compared to standards of Chinese Pharmacopeoia, national standards for pollution-free vegetables and some other Standards, contents of heavy metal elements were lower. Conclutions: Jujube samples were rich in amino acids and trace elements. The research provided useful reference for further research. Keywords: Amino acids; trace elements; inductively coupled plasma - mass Chinese Library Classification(CLC): R284.1 Document code?: A Article ID: 自古以来，人们一直把红枣视作上等滋补品，红枣有非常好的医疗功能，常作百药之引，誉有“红娘”之称。《本草纲目》记载:“熟则可食，干则可补，丰俭可以剂时，疾苦可以备药，辅助粮食以养民生”，因此红枣在我国被列为药食同源植物中首选的保健食品。 我国是红枣主产区，产量约占世界总产量的90%以上，其中山西省吕梁地区红枣种植面积已发展到166万亩，正常年景产量1.2亿千克以上[1]。 据近现代研究表明，枣果中含16 种氨基酸，其中有8 种人体必需的氨基酸和幼儿不能合成的精氨酸和组氨酸[2]。另外还含有包括硒在内的36种微量元