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ARTICLE IN PRESS Insect Biochemistry and Molecular Biology 38 (2008) 788– 795 Contents lists available at ScienceDirect Insect Biochemistry and Molecular Biology journal homepage: /locate/ibmb Regulation of soluble and membrane-bound trehalase activity and expression of the enzyme in the larval midgut of the bamboo borer Omphisa fuscidentalis Nujira Tatun a, Tippawan Singtripop a,, Jatuporn Tungjitwitayakul a, Sho Sakurai b, a Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand b Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan a r t i c l e i n f o a b s t r a c t Article history: Diapausing larvae of Omphisa fuscidentalis contain soluble and membrane-bound trehalase in the Received 30 January 2008 midgut. Soluble trehalase activity accounts for three-fourths of the total trehalase activity in midgut Received in revised form homogenates. The exposure of diapausing larvae to juvenile hormone analog (JHA) induced pupation, 14 April 2008 accompanied by an increase in soluble trehalase activity at the beginning of the prepupal period. Accepted 15 May 2008 Injection of 20-hydroxyecdysone (20E) increased the level of soluble trehalase activity 5 days postinjection in a dose-dependent manner. In contrast, no increase i