补肾壮骨配方颗粒的HPLC 指纹图谱研究Δ.pdfVIP

琛ヨ偩澹閰嶆柟棰楃矑鐨凥PLC 鎸囩汗鍥捐氨鐮旂┒螖 锛 # 闄堣鎰 锛岄煩涓借悕 锛岄倱浼熸皯锛堝箍宸炲啗鍖哄箍宸炴€诲尰闄㈣嵂鍓傜锛屽箍宸 510010锛 + 涓浘鍒嗙被鍙 R283.627锛汻917 鏂囩尞鏍囧織鐮 A 鏂囩珷缂栧彿 1001-0408锛?013锛?3-4075-03 DOI 10.6039/j.issn. 1001-0408.2013.43.15 鎽 瑕 鐩殑锛氬缓绔嬭ˉ鑲惧．楠ㄩ厤鏂归绮掔殑鎸囩汗鍥捐氨銆傛柟娉曪細閲囩敤楂樻晥娑茬浉鑹茶氨锛圚PLC 锛夋硶銆傝壊璋辨煴涓篒nertsil C18锛?50mm脳4.6mm 锛 5渭m 锛夛紝娴佸姩鐩镐负涔欒厛-姘达紙姊害娲楄劚锛夛紝娴侀€熶负1ml/min 锛屾娴嬫尝闀夸负285nm 銆傞噰鐢ㄧ洿瑙傚垎鏋愬拰鐩镐技搴﹁蒋浠惰瘎浠?0鎵硅ˉ鑲惧．楠ㄩ厤鏂 棰楃矑鎸囩汗鍥捐氨鐨勭浉浼煎害銆傜粨鏋滐細寤虹珛浜嗚ˉ鑲惧．楠ㄩ厤鏂归绮掔殑鎸囩汗鍥捐氨锛?0鎵规牱鍝佸叡鏍囧畾浜?涓叡鏈夊嘲锛屽悇鑹茶氨宄板垎绂诲害杈冨ソ銆佺浉浼 搴﹁緝楂橈紝绗﹀悎涓嵂鑹茶氨鎸囩汗鍥捐氨鐮旂┒鐨勬妧鏈姹傘€傜粨璁猴細璇ユ柟娉曞噯纭€佸彲闈狅紝寤虹珛鐨凥PLC 鎸囩汗鍥捐氨鍙负琛ヨ偩澹閰嶆柟棰楃矑鐨勮川閲 鎺у埗鎻愪緵绉戝鍑嗙‘鐨勪緷鎹€ 鍏抽敭璇 琛ヨ偩澹閰嶆柟棰楃矑锛涙寚绾瑰浘璋憋紱楂樻晥娑茬浉鑹茶氨娉 Study on HPLC Fingerprints of Bushen Zhuanggu Formula Granules CHEN Xing-yu 锛孒AN Li-ping 锛孌ENG Wei-min 锛圖ept. of Pharmacy 锛孏uangzhou General Hospital of Guangzhou Military Command 锛孏uangzhou 510010锛孋hina 锛 ABSTRACT OBJECTIVE 锛歍o establish HPLC fingerprint of Bushen zhuanggu formula granules. METHODS 锛欻PLC method was adopted. The separation was performed on Inertsil C18锛?50mm脳4.6mm 锛? 渭m 锛塩olumn with mobile phase consisted of acetoni- trile-water 锛坓radient elution 锛塧t the flow rate of 1ml/min. The detection wavelength was set at 285nm. The fingerprint similarity of 10batches of Bushen zhuanggu formula granules was evaluated by visual analysis and similarity software. RESULTS 锛欶ingerprint spectrum of Bushen zhuanggu formula granules was established. 10batches of samples were detected and 9 peaks in the chromato- gram were common. There was a high similarity and each chromatographic peak was obtained with good separation 锛寃hich were in line with the technical requirements of fingerprint of TCM. CONCLUSIONS 锛歍his method is accurate and reliable 锛宎nd HPLC fin- gerprint provides a scientific basis for the quality control of Bushen zhuanggu formula granules. KEY WORDS Bushen zhuanggu formula granules 锛汧ingerprint 锛汬PLC