流式细胞仪基本原理,技术要点及应用.pdf

北京达科为生物技术有限公司 技术部: 杨亚军 Why Use Flow Cytometry? • The use of flow in research has boomed since the mid-1980s • Contributed to 140,000 published articles (Pubmed) in 2014 16000 s n o i t 12000 a c i l b u 8000 P f o # 4000 1950 1960 1970 1980 1990 2000 2010 2020 Westmead Millennium Institute Outline • Learn the basic principles of flow. • Understand how a flow cytometer works. • Learn how data is interpreted. • Flow Cytometry Applications. What Is Flow Cytometry? • Flow ~ motion • Cyto ~ cell • Metry ~ measure Measuring properties of individual cells while in a fluid stream (flowing) Including size, complexity, phenotypes (surface and intracellualr proteins) viability What is Flow Cytometer? Electronics Fluidics computer Optics Lasers Detectors l Fluidics System Fluidics System Flow Chamber Sample Sheath Sheath laser Sheath versus sample pressure Normally, we keep the sheath pressure FIXED, and adjust the sample pressure . SHEATH SHEATH PRESSURE PRESSURE Cells too close!