MALDI-TOF质谱分析法.PDF

領 不 來 易 不易 便 論 利雷 離行 奈金 量 洛 糖 糖 糖 糖 糖 行串聯 量 易 靈 度度 糖量 雷 離 行 奈金 洛糖 I Abstract Drug metabelism plays an important role in the field of biology and medicine, because the drug metabolites along with the drug itself are absorbed and affect the body physiology after medicine administration. Therefore the investigation of drug metabolism and the analysis of drug metabolites investigation of drug metabolism and the analysis of drug metabolites become a very important item in the pharmacology. In this study, a nano-gold assisted laser desorption/ionization time-of-flight mass spectrometry(NALDI-TOF-MS) for statin drugs and carbohydrate analysis in the serum was developed. Owing to the novel characteristics of nano-gold colloid, a very clean mass spectrim was obtained in contranst with the conventional matrix assisted laser desorption ionization time-of-flight mass spectrometry, in which various confusing peaks resulted from conventional matrix fragmentation always appear and mix with the analyte peaks in the sub 1000 Da range. Finally,a good calibration cure was also established for the quantitative analysis of serum glucose in the range of 1mM to 10mM by NALDI-TOF MS. Keywords laser desorption time-of-flight nano-gold colloid mevinolinglucosematrix background interference II 了 路不 了 練 了