细胞工程实与验技术.pptVIP

10/2005 CE Lecture 1细胞工程实验室基本实验技术 细胞培养的基本概念 细胞培养的基本条件 细胞培养的基本技术 紫外线消毒主要用于培养室空气、操作台、塑料培养　　　　　　皿和培养板等表面消毒 Pipettes, bottles, flasks, petri dishes ? 塑料 -- better attachment and cell growth of monolayer cell culture -- more expensive than reusable glass -- suitable for storage at 4oC ? 玻璃 -- Can withstand temperatures 0oC use for maintaining frozen stocks -- Steam sterilize by autoclaving * relies on steam pressure and high heat * use fast-dry cycle to dry condensate * use “wet” cycle for salt solutions to prevent evaporation * loosely place caps on bottles 实验室安全 培养基的成分及配置 细胞培养的基本操作 Filtr