MTX 与DNA相互作用.pdf

Biosensors and Bioelectronics 44 (2013) 21–26 Contents lists available at SciVerse ScienceDirect Biosensors and Bioelectronics journal homepage: /locate/bios Interaction of anticancer drug methotrexate with DNA analyzed by electrochemical and spectroscopic methods Bushra Rafique a, Ahmad M. Khalid b,n, Kalsoom Akhtar c, Abdul Jabbar a a Department of Chemistry, Government College University, Faisalabad, Pakistan b School of Biological Sciences, University of Sargodha, Sargodha, Pakistan c Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan a r t i c l e i n f o a b s t r a c t Article history: Electrochemical DNA biosensor was used to study the interaction of methotrexate (MTX) with DNA Received 26 October 2012 immobilized on the bare surface of glassy carbon electrode (GCE). The binding mechanism of MTX with Received in revised form DNA was elucidated by using constant current potentiometric technique further supported by UV– 13 December 2012 Visible and FT-IR studies. The decrease in guanine peak area was used as an analytical signal for the Accepted 14 December 2012 Available online 27 December 2012 interaction of drug with DNA in acetate buffer solution at pH 4.2 (20% ethanol). The binding constant (K ) value calculated for MTX was 3.821 105 M 1. UV–Visible studies indicated hyperchromic and Keywords: hypsochromic shifts in the maximum absorption bands of MTX after i