JAK2STAT3 通路在表皮生长因子you导结肠癌细胞侵袭迁移中的作用.docVIP

JAK2-STAT3通路在表皮生长因子诱导结肠癌细胞侵袭迁移中的作用 徐建华，张 超＊ 郝迎学，刘 涛，陈 军，崔 浩（400038重庆,第三军医大学西南医院全军普通外科专科中心，微创胃肠外科中心） 【摘要】　目的　探讨JAK2-STAT3通路在表皮生长因子 (英文全称？EGF)诱导结肠癌细胞侵袭迁移中的作用。方法　以人结肠癌细胞株LOVO 为研究对象, 分为对照组、表皮生长因子处理组（EGF组）、表皮生长因子与JAK2 激酶抑制剂AG490共处理组（EGF+AG490组）。用免疫荧光和Western blot 检测各组LOVO细胞E钙粘素蛋白, 磷酸化STAT3表达水平；Transwell法检测细胞侵袭能力；细胞划痕实验检测细胞迁移能力。结果 与EGF+AG490组结肠癌LOVO细胞相比，EGF组细胞P-STAT3表达增加，E-Cadherin蛋白表达明显降低并由细胞膜向细胞浆内移位，同时侵袭和迁移能力增加（P0.05）。结论　EGF可能通过JAK2-STAT3通路调节E-Cadherin在结肠癌LOVO细胞中的表达和定位，从而增强结肠癌细胞的侵袭迁移能力。 【关键词】　结肠癌；表皮生长因子 ;JAK2/STAT3通路;E钙粘素 The Role of JAK2-STAT3 Pathway in EGF Induce Invasion and Migration of Human Colon Cancer Cells Xu Jian-hua, Zhang Chao, HAO Yinxue ,Liu Tao, Chen Jun, Cui Hao .Department of General Surgery ,Southwest Hospital,The Third Military Medical University,Chongqing 400038,China 【Abstract】　Objective　To investigate the role of JAK2-STAT3 pathway in EGF induce invasion and migration of human colon cancer cells . Methods　The human colon cancer cell LOVO was used as the research object, divide into three groups: control group， treatment with EGF（EGF group）, treatment with EGF and AG490（EGF+AG490 group）. The migration of LOVO cell was measured with scratch assay,and invasion was measured by transwell.The P-STAT3 and E-Cadherin protein in cell was detected by Western-blot and immunofluorescence. Results Compared with the control and EGF+AG490 group, EGF increased the expression of P-STAT3 ,decreased the expression of E-Cadherin and located into cytolymph, and increased the migration and invasion of human colon cancer cells (P 0.05). Conclusion 　 EGF could regulate the expression and location of E-cadherin in human colon cancer cell LOVO through JAK2/STAT3 pathway, and increased the migration and invasion of human colon cancer cells. 【Key words】Colon cancer; EGF ;JAK2/STAT3 ；E-Cadherin Corresponding author: Zhang Chao，E-mail zhangchao5642@163.com 基金项目：全军医学科研“十一五”计划面上项目（06MB243）；重庆市自然科学基金(CSTC 2005BB5291) 请补充基金英文。 通信作者：张 超，E-mail zhangchao5642@163.com 结肠癌是