芸香挥发油 GC-MS 分析及其生物活性研究.PDFVIP

芸香挥发油 GC-MS 分析及其生物活性研究 唐祖年，杨月，杨扬，徐雅娟(桂林医学院，广西 桂林 541004) 摘要：目的 研究芸香挥发油的化学成分、抑菌活性和对HepG2 细胞、NCI-H460 细胞增殖的抑制作用。方法 采用 水蒸气蒸馏法(SD)提取挥发油，通过气相色谱-质谱联用技术(GC-MS)分析芸香挥发油的化学成分，同时采用纸片法测定 其抑菌活性和MTT 法观察芸香挥发油对肝癌HepG2 细胞和肺癌NCI-H460 细胞增殖的抑制作用。结果 从芸香挥发 油共鉴定出21 种成分，主要为 2-十一酮(46.15%) ，其次是2- 壬酮(27.01%)及 2-十三醇乙酸酯(12.73%) ；抗菌实验显 示，芸香挥发油对金黄色葡萄球菌、白色葡萄球菌、表皮葡萄球菌、乙型链球菌有较强的抑菌活性；对肝癌HepG2 细胞和肺癌NCI-H460 细胞的增殖也有抑制作用，48 h 的作用强于24 h ，IC50 分别为23.21 µg·mL1 和21.87 µg·mL1 。 结论 芸香挥发油可作为一种潜在的天然抗菌和抗肿瘤药物的来源。 关键词：芸香；挥发油；气相色谱-质谱联用；抑菌活性；细胞增殖的抑制作用 中图分类号：R284.1 ；R285.5 文献标志码：A 文章编号：1007-7693(2011)09-0834-05 Chemical Composition and Biological Activity of the Essential Oil of Ruta graveolens TANG Zunian, YANG Yue, YANG Yang, XU Yajuan(College of Pharmacy, Guilin Medical College, Guilin 541004, China) ABSTRACT: OBJECTIVE The chemical composition, antimicrobial activity and inhibitory effect to cell proliferation of the essential oil from Ruta graveolens were studied. METHODS The chemical composition of the essential oil obtained by hydrodistillation from Ruta graveolens was analysed by GC-MS. The antimicrobial activity of the oil was evaluated against 9 micro-organisms using agar disc diffusion method. The inhibitory effect of the oil to cell proliferation was evaluated on HepG2 and NCI-H460 cell lines by MTT assay. RESULTS Twenty-one components were identified in the oil, and main compounds of 作者简介：唐祖年，男，副教授 Tel:E-mail: tangzunian@ ·834 · Chin JMAP, 2011 September, Vol.28 No.9 中国现代应用药学2011 年 9 月第 28 卷第 9 期 the oil were 2-Undecanone (46.15%), 2-Nonanone (27.01%) and 2-Acetoxytridecane(12.73%). The oil presented a broad antimicrobial spectrum, and exerted strong bactericidal activity agains Staphylococcus aureus CCTCC 26001, Staphylococcus al