高通量测序技术在黄连解毒汤抗白念珠菌机制研究中的应用和探讨.pdfVIP

环球中医药2013年1月第6卷第1期摇 Global Traditional Chinese Medicine,January2013,Vol郾6,No郾1 摇摇摇55 ·综述 · 高通量测序技术在黄连解毒汤抗白念珠菌 机制研究中的应用及探讨 曹毅摇 翁鹤摇 杨晓红 揖摘要铱摇 白念珠菌(Candida albicans)是临床上发生最为普遍的致病真菌,严重时可危及生命, 目前主要以三唑类药物进行防治。 但是由于药物长期广泛的使用及其作用位点的单一性,导致耐 药问题日益突出。 中药黄连解毒汤有良好的抑菌效果,并极大增强西药杀菌作用,但至今相关其分 子机制的研究鲜有涉及。 本文在阐述目前国内外现有高通量基因测序技术的基础上,重点探讨了 高通量技术在黄连解毒汤抗白色念珠菌机制研究中的应用。 揖关键词铱摇 白念珠菌;摇 黄连解毒汤;摇 高通量测序技术;摇 应用探讨 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10.3969/ j.issn.1674鄄1749.2013.01.020 Application and exploration of high鄄throughput sequencing thechnologies in mechanism study of Huanglian鄄Jiedu Decoction antagonize Candida albicans摇 CAO Yi, WENG He, YANG Xiao鄄hong. Department of Dermatology, First Affiliated Hospital of Zhejiang Chinese Medicine University,Hangzhou 310006,China Corresponding author:CAO Yi,E鄄mail:caoyi1965@163.com 揖Abstract铱摇 Candida albicans is the most common clinical pathogenicfungus and life鄄threatening in serious situations. Triazole is the main drug used in clinic to prevent and treat individuals infected with Candida albicans presently. However, due to long鄄term extensive usage and single site of action, drug resistance problems have become increasingly prominent. Antimicrobial activity of Huanglian鄄Jiedu Decoction has been confirmed,and Huanglian鄄Jiedu Decoction has effective role in enhancing sterilization action ofwesternmedicine,butthemolecularmechanisminwhichisrarelykown. Inthisarticlewediscuss the current situation of the high鄄throughput sequencing thechnologies in the world, and focuse on the application and exploration of high鄄throughput sequencing thechnologies in the mechanism study of Huanglian鄄Jiedu Decoction antagonize Candida albicans. 揖Key words铱摇 Candida albicans;摇 Huanglian鄄Jiedu Decoction;摇 High鄄throughput sequencing thechnology;摇 Application and discussion