组织培养对沙柳器官形成与植株 再生的影响研究.pdf

Botanical Research 植物学研究, 2017, 6(5), 282-292 Published Online September 2017 in Hans. /journal/br /10.12677/br.2017.65037 Effect of Tissue Culture Conditions on Organogenesis and Replant of Salix psammophila 1 2 1 1 1 2 Lijun He , Haijun Chen , Xianwei Wu , Jiawei Liu , Kebin Mu , Jie Hou 1 Agricultural College, Inner Mongolia Agricultural University, Huhhot Inner Mongolia 2 Inner Mongolia Institute of Biotechnology, Huhhot Inner Mongolia th th th Received: Aug. 20 , 2017; accepted: Sep. 4 , 2017; published: Sep. 8 , 2017 Abstract Salix psammophila is one of the important eco-economic plants in northern arid and desert region. In this experiment, on the basis of the strong tender stem segments, the key skills of how regene- rated plant forms in different Salix pasammophila tissue culture conditions were explored, by means of tissue culture to get the organs formed and differentiated and to induce regeneration plants formed in succession. Research results show that Salix psammophila gets better bud diffe- rentiation effect in MS medium, the suitable differentiation and proliferation medium is MS + 0.5 mg/L 6-BA + 0.1 mg/L NAA; the subculture medium is MS + 0.2 mg/L 6-BA + 0.2mg/L KT + 0.1 mg/L NAA, with shifting the culture with the medium MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA, a me- dium with little higher hormone, to achieve the best effect; the rooting medium is 1/2MS + 0.3 mg/L IBA + 0.05 mg/L NAA, all the results establish the regeneration system. This method not only provides the materials source for Salix