茉莉酸甲酯对红桧叶子挥发成分含量之影响-国立中兴大学试验林管理处.pdfVIP

34(1) 83-96, 2012 83 林業研究季刊 ： 研究報告 茉莉酸甲酯對紅檜葉子揮發成分含量之影響 1 2 1,3,4,* 郭佩旻　曲芳華 　王升陽 【摘要】紅檜為台灣特有樹種，木材具有極佳的耐久性並具特殊的香味。為瞭解紅檜葉子的揮發性 成分組成，本研究除利用水蒸氣蒸餾法製備精油外，並利用固相微萃取技術收集葉子的揮發性成 分，續以氣相層析質譜鑑定成分。此外，本研究並以茉莉酸甲酯模擬葉子受到機械創傷或是昆蟲、 108 真菌的侵害後，其揮發性成分的變化。由分析結果可知，共 種紅檜葉子之化合物被鑑定出，在 揮發性成分中以α-Pinene為最主要成分，佔30.29% ，而在精油中則是以β-Myrcene為主成分，佔 22.45% 。紅檜葉子在茉莉酸甲酯處理後，其揮發性成分之釋出量大幅提高，揮發量為未以茉莉酸甲 1.92 10 mM 酯處理的葉子的 倍；而在茉莉酸甲酯處理後對於時間上的影響上，在施加 茉莉酸甲酯經 5 hr 過 後，其揮發量增加最多，之後揮發量就開始減少。 【關鍵詞】紅檜、揮發成分、精油、茉莉酸甲酯 Research paper Effect of Methyl Jasmonate on the Amounts of Volatile Compounds Emitted from Chamaecyparis formosensis Matsum. Leaves Pei-Min Kuo 1　Fang-Hua Chu2　Sheng-Yang Wang1,3,4,* Abstract Chamaecyparis formosensis is an endemic tree grown in Taiwan. The durability of C. 【 】 formosensis wood is excellent and with fragrance odor. In order to analysis the volatile compounds of C. formosensis , in addition to the essential oil of leaves was prepared by water distillation, the solid phase micro extraction technology was also conducted to collect the compounds emitted from leaves. Moreover, the leaves of C. formosensis were treated by methyl jasmonate (MeJA) to mimic it wounded or attacked by fungus or insects. According to the results obtained in our study, totally 108 compounds were identified 1. 國立中興大學森林學系 Department of Forestry, National Chung-Hsing University, Taichung, Taiwan 2. 國立台灣大學森林環境暨資源學系 School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan 3. 國立中興大學實驗林管理處 Experimental Forest, National Chung-Hsing Univers