氯化钴后处理对新生鼠缺氧缺血性脑损伤远期空间-第三军医大学学报.docVIP

氯化钴后处理对新生鼠缺氧缺血脑损伤学习记忆的作用 李文迪2，钟敏3，刘友学2，陈洁2，程茜1，李廷玉1，代英1 重庆医科大学大学附属儿童医院1儿童保健科2儿3神经内科, 重庆400014摘要】 探讨氯化钴(CoCl2)后处理对缺氧缺血性脑损伤新生大鼠空间学习记忆的作用。 生后7日龄SD大鼠66只，分为假手术组(N组，n=16)、缺氧缺血组(HI组，n=18)、CoCl2即刻干预组(C1组，n=14)、CoCl2术后1d干预组(C2组，n=18)。Western blot检测术后1d、2d、7d脑组织HIF-1α蛋白的表达。大鼠生后7周行水迷宫观察CoCl2对大鼠空间学习记忆的影响。 HI组和C1组HIF-1α蛋白在术后第一天、第二天增多,第七天则不能检测到HIF-1α蛋白。而C2组第七天仍能检测到HIF-1α蛋白。水迷宫实验显示C2组较HI组3~ 5 d时平均潜伏时间明显缩短穿越原平台区域的时间增加，空间学习记忆能力部分恢复 (p 0.05)。 在新生鼠缺氧缺血性脑损伤时, CoCl2HIF-1α蛋白的表达,术后1天干预可有效恢复大鼠的空间学习记忆能力。 【关键词】：缺氧诱导因子；氯化钴；缺氧缺血性脑损伤；空间学习记忆 【中图法分类号】: R722.12;R741.05 【文献标识码】：A Cobalt Chloride Postconditioning on Rat Model for Perinatal Hypoxic-Ischemic Encephalopathy Li Wendi 2 ,Zhong Min 3, Chen Jie 2，, Liu Youxue 2, Cheng Qian 1, Tingyu Li1,2, Ying Dai1 1 Department of Primary Child Health care 2 Childrens Nutritional Research Center, Key Laboratory of Developmental Diseases in Childhood of Education Ministry, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorder 3 Department of Neurology, Children’s Hospital of Chongqing Medical University Chongqing ,400014 【Abstracts】 Objective To investigate the optimal time to improve the spatial working memory by cobalt chloride (CoCl2) postconditioning in hypoxic-ischemic induced brain damage(HIBD) rat. Methods Sixty-six SD rats aged 7 days were randomly divided into sham operation group (N，n=16), hypoxia-ischemia group (HI, n=18), CoCl2 administration immediately after surgeries group (C1, n=14) and CoCl2 administration 1d after surgeries group (C2, n=18). The left common carotid artery of rats were ligated after anesthesia followed by hypoxia. In the sham control group, the left common carotid artery was exposed but was not ligated or exposed hypoxia. The brain tissues were harvested from the pups in each group at 1, 2 and 7 days after insult. The HIF-1α protein expression was detected by Western blot analysis. At age of 7 weeks,