植物乳杆菌细胞壁肽聚糖的微胶囊化.pdfVIP

※基础研究 食品科学 2011, Vol. 32, No. 17 27 朱 丽，韩德权，孙庆申* ，吴 桐，李昊哲 (黑龙江大学生命科学学院，微生物黑龙江省高校重点实验室，黑龙江 哈尔滨 150080) 以壳聚糖(CS)为壁材，采用乳化交联法制备载植物乳杆菌细胞壁肽聚糖(PG) 的CS 微球，并通过动物实验 研究口服 PG 微球的缓释效果。通过低、中、高 3 种剂量裸 PG 和 PG 微球对小鼠进行灌胃 1 周处理，每隔 3d 断尾 取血，连续进行 15d，ELISA 法检测小鼠血清中溶菌酶含量。结果表明：微球的载药量和包封率依壁、芯材比 而变化，其中，PG 和 CS 质量比为 1:1 的复合微球平均载药量为(37.00 ±3.24)g/100g ，包封率为(92.00 ±2.38)% 。 在免疫后第 9 天各微球组小鼠血清中溶菌酶含量均达到最高值，明显高于对照组，免疫后第 15 天各微球组血清中 溶菌酶含量仍保持较高水平。载 PG 的CS 微球具有显著的缓释效果，能够显著延长 PG 作用时间。 肽聚糖；壳聚糖；微球；缓释 Microcapsulation of Cytoderm Peptidoglycan Derived from Lactobacillus plantarum ZHU Li ，HAN De-quan ，SUN Qing-shen*，WU Tong ，LI Hao-zhe (University Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China) Abstract：Chitosan microspheres encapsulating cytoderm peptidoglycan derived from Lactobacillus plantarum were prepared by emulsion-crosslinking method. The sustained-release effect of peptidoglycan was evaluated in experimental mice orally administered with naked peptidoglycan or peptidoglycan microspheres for a consecutive week at low, medium and high dosages. Blood was collected from mouse tails within 15 d at an interval of 3 d. Enzyme-linked immunosorbent assay (ELISA) was used to measure lysozyme content in mouse serum. The results showed that the average drug loading capacity (LC) and encapsulation efficiency (EE) of microspheres varied with different ratios between peptidoglycan and chitosan. At the ratio of 1:1, the LC and EE were( 37.00 ± 3.24) g/ 100 g and (92.00 ± 2.38)%, respectively. The highest lysozyme content in serum was achieved at the 9th day post-administration of peptidoglycan microspheres, which was significantly higher than that in the control group. Meanwhil