虾蛤蚌中四环素耐药菌及四环素耐药基因的研究-现代食品科技.pdfVIP

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.10 广州市售鱼、虾、蛤蚌中四环素耐药菌及四环素 耐药基因的研究 余丽，熊丽娜，石磊，叶蕾 （华南理工大学轻工与食品学院，广东广州 510640） 摘要：本文以广州市大型超市的鱼、虾、蛤蚌为研究对象，研究其四环素耐药菌的分布及四环素耐药基因的携带情况。采用平 2 6 6 板涂布法分离四环素耐药菌并统计菌落数，计数结果在 10 ~10 CFU/g 之间，其中鱼肠中四环素耐药菌的数量达 10 CFU/g 。通过 PCR 对样本总 DNA 中四环素耐药基因的携带情况进行检测，发现每个样本均携带多个（3~7 个）四环素耐药基因。9 种四环素耐药基因 中，tet(E)的检出率最高6.3%，其次分别为tet(S) (5.1%)，tet(M) (3.1%)，tet(C) (1.7%)和 tet(G) (0.9%)。Southern 杂交试验表明四环素 抗性菌株Aeromonas spp.和Escherichia coli 携带的耐药基因 tet(E)存在于质粒上；在无四环素选择压力下对上述两种菌株进行连续传 代，其耐药质粒仍稳定存在于菌株中。本研究表明广州市售鱼、虾、蛤蚌中分离得到的细菌对四环素耐药情况较为严重，应得到相关 监管部门以及消费者的足够重视。 关键词：水产品；四环素耐药性；质粒 文章篇号：1673-9078(2016)10-239-245 DOI: 10.13982/j.mfst.1673-9078.2016.10.036 Tetracycline-resistant Bacteria and Tetracycline-resistant Genes from Fish, Shrimp, and Clams in Guangzhou YU Li, XIONG Li-na, SHI Lei, YE Lei (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: Aquatic products (fish, shrimps, and clams) from markets in Guangzhou (Guangdong Province) were tested to determine the presence and distribution of tetracycline (Tet)-resistant (Tetr) genes and bacterial species. The plate-spread method was employed to isolate Tet-resistant bacterial species, allowing detection of 102 6 r r 6 CFU to 10 CFU/g Tet bacteria, with the highest number of Tet -bacteria counts (10 CFU/g) detected in fish intestines. Conventional polymerase chain reaction (PCR) was conducted o